Development of an ultra sensitive UPLC-SRM/MS method for quantification of urinary eicosanoids

 

Eicosanoids are key mediators and regulators of inflammation and oxidative stress often used as biomarkers for diseases and pathological conditions such as cardiovascular and pulmonary diseases, as well as cancer.

Key urinary eicosanoid species related to pro-inflammatory states or oxidative stress are the following:

 

(A) Tetranor PGE-M

(B) 8-iso-PGF2a (also named 8-epi-PGF2 a, 8-isoprostane or iPF2 a -III),

(C) 2,3-dinor-8-iso-PGF2 a

(D) 2,3-dinor-TXB2

(E) 11-dehydro-TXB2

(F) LTE4

(G) 12-HETE

 

Although there are existing methods for profiling of single urinary eicosanoid classes/species, methods for quantification of urinary eicosanoid species generated from different pathways are rare.

Therefore we developed a simple, robust and sensitive method for quantification of urinary eicosanoid species generated from different pathways (for the metabolites described above; A-G) applicable in clinical studies.

The novel method was validated in human urine and has been applied to a series of smoker urine samples, as it is known that cigarette smoking and nicotine consume lead to elevated urinary levels of eicosanoids with pro-inflammatory effects.

 

The method was published in the May 2012 issue of "Journal of Lipid Research"

(2010 Impact Factor: 6.115).

 

JLR Issue

 

Related publication:

Sterz C, Scherer G, Ecker J. (2012) „A simple and robust UPLC-SRM/MS method to quantify urinary eicosanoids“ JLR 53(5) 1026-1036

 

The method robustness has already been proven in our lab in a recent application on several hundred samples of a nutrition study.

Please This e-mail address is being protected from spambots. You need JavaScript enabled to view it. for more information.

 

Josef Ecker

23rd April 2012