Fatty acid identifcation with GC-TOF - New publication


Conjugated fatty acids (CFAs) exhibit growth inhibitory effects on colon cancer in vitro and in vivo. CFAs can be found in dairy products, meat and vegetable oils. The study from Degen et al., shows that the anticancerogenic potency of CFAs depends on number or configuration of the conjugated double bonds and that these fatty acids are metabolized by HT-29 cells.

Using time- and dose-dependent stimulation experiments several new peaks within the GC chromatograms and thus metabolites could be found. These new metabolites/CFAs were identified by their fragment patterns determined with GC-TOF at ABF. The advantage of the used TOF mass spectrometer over a conventional quadrupole mass spectrometer is its higher resolution.

With this study the authors could identifiy novel CFA metabolites and show that growth inhibition of human cancer cells is associated with a specific cellular metabolic profile of fatty acid metabolism contributing to the diversified ability of CFAs as anti-cancer compounds.

Related publication:

Degen C, Ecker J, Piegholdt S, Liebisch G, Schmitz G, Jahreis G. (2011) "Metabolic and growth inhibitory effects of conjugated fatty acids in the cell line HT-29 with special regard to the conversion of t11,t13-CLA." Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1811(12), 1070-80

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Josef Ecker

19th October 2011