"Two enzymes (COX-2 and 5-LOX) crossing the boarder" - Novel 5-S-HETE derived eicosanoids identified

 

Biosynthesis of novel eicosanoids - HKD2 and HKE2


Eicosanoids are lipid mediators with various physiological effects. Prostaglandins and leukotrienes are well known eicosanoids generated by two distinct pathways by cyclooxygenase-2 (COX-2) and 5-lipoxygenease (5-LOX).


Griesser and co-workers present data in a recent PNAS paper that both enzymes, COX-2 and 5-LOX, are involved in the formation of novel hemiketal eicosanoids (HKD2, HKE2) from 5-S-HETE. The work further shows that these metabolites are produced by human blood leukocytes activated with lipopolysacharide (LPS). Because HKED2 and HKE2 stimulate migration and tubulogenesis of microvascular endothelial cells, it is proposed that these compounds might have a proangiogenic role at inflammatory sites.

HKE2 and HKD2 have been identified with LC-MS and NMR approaches.

 


Griesser M, Suzuki T, Tejera N, Mont S, Boeglin WE, Pozzi A, Schneider C. (2011), "Biosynthesis of hemiketal eicosanoids by cross-over of the 5-lipoxygenase and cyclooxygenase-2 pathways". PNAS 108(17): 6945-50

 

 

Josef Ecker

15th November 2011