Tobacco Harm Reduction

Cigarette smoking remains the leading cause of preventable premature death and disease. From conventional cigarettes to non-combustible alternatives, at ABF we help to understand the continuum of risk associated with nicotine-containing products. Our validated bioanalytical methods provide reliable data for regulatory submissions.

Our Commitment

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Extensive expertise in the analysis of biological samples for both clinical and non-clinical studies

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Uncompromising Quality, Compliance, and Data Integrity

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Timely and Accurate Analysis & Reporting Supporting Decision-Making and Research Efforts

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Expert Bioanalytic Services for Regulated Biomarker Studies

Nicotine Pharmacokinetic Analysis for Regulatory Submissions

Trusted Bioanalytical Services Tailored to Your Specific Application

Integrated Solutions for Seamless Sample Management World Wide

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We would be happy to advise and support you with your project or your study. Send us your request and tell us your specific wishes.

Our biomarker methods

Biomarker
Exposure to
Matrix
Analytical Technique
Reference
11-dehydro-TXB2 Inflammation Urine LC-MS/MS
12(S)-HETE Inflammation Urine LC-MS/MS
1,3-Butadiene Tobacco Smoke ETS GC-MS
1-Aminonaphthalene (1-AN) 1-Aminonaphthalene Urine GC-MS
1-Hydroxyphenanthrene (1-OH-Phe) Phenanthrene Urine LC-MS/MS
1-Hydroxypyrene (1-OH-Pyr) Pyrene Urine LC-MS/MS
1-Naphthol (1-Hydroxynaphthalene, 1-OH-Nap) Naphthalene Urine LC-MS/MS
20-Carboxy-Leukotriene B4 Inflammation Urine LC-MS/MS
20-Hydroxy-Leukotriene B4 Inflammation Urine LC-MS/MS
2,3-dinor-8-iso-PGF2α Oxidative Stress Urine LC-MS/MS
2,3-dinor-TXB2 Inflammation Urine LC-MS/MS
2-Aminonaphthalene (2-AN) 2-Aminonaphthalene Urine, ETS GC-MS
2-(Carboxyl-1-methylethyl)mercapturic acid (CMEMA) Crotonaldehyde Urine LC-MS/MS
2-Hydroxy-1-phenylethylmercapturic acid (PHEMA 1) Styrene Urine LC-MS/MS
2-Hydroxy-2-phenylethylmercapturic acid (PHEMA 2) Styrene Urine LC-MS/MS
2-Hydroxyethylmercapturic acid (HEMA) Ethylene oxide, Alkylating agents Urine LC-MS/MS
2-Hydroxyfluorene (2-OH-Flu) Fluorene Urine LC-MS/MS
2-Hydroxyphenanthrene (2-OH-Phe) Phenanthrene Urine LC-MS/MS
2-Hydroxypropylmercapturic acid (2-HPMA) Propylene oxide Urine LC-MS/MS
2-Naphthol (2-Hydroxynaphthalene, 2-OH-Nap) Naphthalene Urine LC-MS/MS
3-Aminobiphenyl (3-ABP) 3-Aminobiphenyl Urine GC-MS
3-Ethenylpyridine Tobacco Smoke Environmental Tobacco Smoke (ETS) GC-MS
3-Hydroxy-1-methylpropylmercapturic acid (HMPMA) Crotonaldehyde Urine LC-MS/MS
3-hydroxybenzo[a]pyrene (3-OH-BaP) Benzo[a]pyrene Urine LC-MS/MS
3-Hydroxyphenanthrene (3-OH-Phe) Phenanthrene Urine LC-MS/MS
3-Hydroxypropylmercapturic acid (3-HPMA) Acrolein Urine LC-MS/MS
3-Nitrotyrosine Nitrosative stress Plasma LC-MS/MS
4-Aminobiphenyl (4-ABP) 4-Aminobiphenyl Urine, ETS GC-MS
4-Hydroxy-4-(3-pyridyl)butanoic acid (HyPyBut) Nicotine Urine LC-MS/MS
4-Hydroxyphenanthrene (4-OH-Phe) Phenanthrene Urine LC-MS/MS
8-Hydroxy-deoxyguanosine (8-OH-dG) Oxidative stress Urine LC-MS/MS
8-iso-PGF2α Oxidative Stress Urine LC-MS/MS
9-Hydroxyphenanthrene (9-OH-Phe) Phenanthrene Urine LC-MS/MS
Acetaldehyde (FA) Tobacco Smoke, E-Vapor Aerosol ETS, e-liquids, aerosol LC-MS/MS
Acetone Tobacco Smoke, E-Vapor Aerosol ETS, e-liquids, aerosol LC-MS/MS
Acrolein (ACR) Tobacco Smoke, E-Vapor Aerosol ETS, e-liquids, aerosol LC-MS/MS
Acrylamidemercapturic acid (AAMA) Acrylamide Urine LC-MS/MS
Anabasine (AB) Anabasine Plasma, Urine LC-MS/MS
Anatabine (AT) Anatabine Plasma, Urine LC-MS/MS
Benzene Tobacco Smoke ETS GC-MS
Benzo[a]pyrene (BaP) PAH ETS GC-MS
Benzo[a]pyrene tetrol (BaP-tetrol) Benzo[a]pyrene Urine GC-MS/MS
Citronellol Flavor Urine HS-SPME-GC-MS
Cotinine Nicotine Urine, Plasma, Serum, Saliva LC-MS/MS
Cotinine-glucuronide Nicotine Urine LC-MS/MS
Cotinine-N-oxide (CNO) Nicotine Urine LC-MS/MS
Creatinine Normalization of urinary biomarker concentrations Urine Photometry
Cyanoethylmercapturic acid (CEMA) Acrylonitrile Urine LC-MS/MS
DHN-MA Oxidative stress, Lipid peroxidation Urine LC-MS/MS
Dihydroxybutenylmercapturic acid (DHBMA) 1,3-Butadiene Urine LC-MS/MS
Dihydroxypropylmercapturic acid (DHPMA) Glycidol Urine LC-MS/MS
Eugenol Flavor Urine HS-SPME-GC-MS
F-PM Tobacco Smoke Environmental Tobacco Smoke (ETS) Fluorescence
Fromaldehyde (FA) Tobacco Smoke, E-Vapor, Aerosol ETS, e-liquids, aerosol LC-MS/MS
Glycerol Glycerol Urine, Plasma, Serum, Saliva, PBS, ETS, Aerosol, e-liquids LC-MS/MS
Glycidamidemercapturic acid (GAMA) Acrylamide Urine LC-MS/MS
HNA-Lactone-MA Oxidative stress, Lipid peroxidation Urine LC-MS/MS
HNA-MA Oxidative stress, Lipid peroxidation Urine LC-MS/MS
HNE-MA Oxidative stress, Lipid peroxidation Urine LC-MS/MS
Homocysteine Amino acid metabolism Plasma LC-MS/MS
Isoprenemercapturic acid (IPMA-3) Isoprene Urine LC-MS/MS
L-Carvone Flavor Urine HS-SPME-GC-MS
Leukotriene E4 Inflammation Urine LC-MS/MS
Linalool Flavor Urine HS-SPME-GC-MS
MDA Oxidative stress, lipid peroxidation Plasma, Exhaled Breath GC-MS
Menthol Flavor Urine HS-SPME-GC-MS
Methacrolein Tobacco Smoke, E-Vapor Aerosol ETS, e-liquids, aerosol LC-MS/MS
Monohydroxybutenylmercapturic acid 1 (1-MHBMA) 1,3-Butadiene Urine LC-MS/MS
Monohydroxybutenylmercapturic acid 2 (2-MHBMA) 1,3-Butadiene Urine LC-MS/MS
Nicotine-glucuronide (Nic-gluc) Nicotine Urine LC-MS/MS
Nicotine (Nic) Nicotine Urine, Plasma, Serum, Saliva, PBS, ETS, e-liquids, aerosol LC-MS/MS, GC-MS
Nicotine-N-oxide (NNO) Nicotine Urine LC-MS/MS
NNAL NNK (Nicotine-derived nitrosamine ketone) Urine LC-MS/MS
N-Nitrosoanabasine (NAB) NAB Urine, ETS, Aerosol LC-MS/MS, GC-MS
N-Nitrosoanatabine (NAT) NAT Urine, ETS, Aerosol LC-MS/MS, GC-MS
N-Nitrosonornicotine (NNN) NNN Urine, Plasma, Saliva, ETS, Aerosol LC-MS/MS, GC-MS
NNK Tobacco Smoke, Aerosol ETS, Aerosol LC-MS/MS
Norcotinine Nicotine Urine LC-MS/MS
Nornicotine Nicotine Urine LC-MS/MS
ortho-Toluidine ortho-Toluidine Urine, ETS GC-MS
para-Menthane-3,8-diol Menthol Plasma, Serum LC-MS/MS
Particulate matter (PM) Tobacco Smoke Environmental Tobacco Smoke (ETS) Light scattering or gravimetry
PGF2α Oxidative Stress Urine LC-MS/MS
Phenantrene (Phe) PAH ETS GC-MS
Propionaldehyde (PA) Tobacco Smoke, E-Vapor Aerosol ETS, e-liquids, aerosol LC-MS/MS
Propylene glycol (PG) Propylene glycol Urine, Plasma, Serum, Saliva, PBS, Aerosol, ETS, e-liquids LC-MS/MS
Prostaglandin D Oxidative Stress Urine LC-MS/MS
Prostaglandin E Oxidative Stress Urine LC-MS/MS
Pyrene (Pyr) PAH ETS GC-MS
S-Benzylmercapturic acid (SBMA) Toluene Urine LC-MS/MS
sICAM-1 Inflammation Plasma ELISA
Solanesol Tobacco Smoke Environmental Tobacco Smoke (ETS) LC-MS
S-Phenylmercapturic acid (SPMA) Benzene Urine LC-MS/MS
Toluene Tobacco Smoke ETS GC-MS
trans-3'-Hydroxycotinine (3-OH-Cot) Nicotine Urine, Plasma, Serum, Saliva LC-MS/MS
trans-3'-Hydroxycotinine-glucuronide (3-OH-Cot-gluc) Nicotine Urine LC-MS/MS
trans,trans-muconic acid (tt-MA) Benzene Urine GC-MS
Trehalose Trehalose PBS LC-MS/MS
UV-PM Tobacco Smoke Environmental Tobacco Smoke (ETS) Photometry
Xylene Tobacco Smoke ETS GC-MS

Pioneers in biomarker research

Almost all of the biomarker methods developed at ABF were published in peer-reviewed journals, a fact that supports the validity, transparency and scientific evidence of the bioanalysis especially of importance for regulatory submissions